To investigate whether caspase-7 participates in caspase-1 cleavage and IL-1β secretion during Brucella abortus infection, we infected BMDMs of C57BL/6 (WT), Casp7−/−, Casp7/1/11−/−, Casp7/Gsdmd−/−, and Gsdmd−/− with Brucella. After 17 h of infection, we evaluated IL-1β secretion in the supernatant of the cells (Figure 1A) and the lysate was properly prepared for the assay of caspase-1 processing by Western blot analysis (Figure 1B). This evidence concerns the gene GSDMD and infection.