PKN1 and pulmonary arterial hypertension: To verify if these top 10 hub genes do contribute to PA remodeling in any PH, we performed qRT-PCR to detect three upregulated candidate genes (WASF1, ARHGEF1, and RB1) and seven downregulated candidate genes (IL1R1, RHOB, DAPK1, TNFAIP6, PKN1, PLOD2, and MYOF), using hypoxia with Sugen-induced PAH classified into group 1 (Figure 7).