Gene mutation is often a key link in tumor progression, and we showed the Top 15 genes associated with NECAP2 expression in the TCGA-LGG dataset with significant differences in mutation frequency (Figure 1(e)), including IDH1 (p = 2.4e − 5), TP53 (p = 3.0e − 22), ATRX (p = 8.1e − 19), CIC (p = 2.1e − 27), TTN (p = 0.10), FUBP1 (p = 1.1e − 11), NOTCH1 (p = 4.1e − 5), PIK3CA (p = 0.17), EGFR (p = 3.0e − 3), PTEN (p = 6.5e − 3), IDH2 (p = 3.6e − 4), ZBTB20 (p = 3.0e − 3), NIPBL (p = 5.0e − 3), KAT6B (p = 0.04), and USP11 (p = 0.04). This evidence concerns the gene NOTCH1 and neoplasm.