CISH and in situ carcinoma: We treated 13 CCA cell lines (YSCCC, KKU213A, KKU213B, KKU100, HuCCA1, KKU055, HuH28, KKU138, HuCCT1, SSP25, RBE, KKU131, and KKU068) with GEM/CIS, LCL161, or LCL161 + GEM/CIS for 5 days.