Although the combination of P16-Light with P14-qPCR may detect both SCNDs overlapping with the CDKN2A CDR and not overlapping with the CDKN2A CDR, the results of our comparison analysis among 139 GC patients showed that detecting CDKN2A SCND by individual P16-Light may be good enough for clinical practice because combination with P14-qPCR could not improve the performance of P16-Light. The gene discussed is CDKN2A; the disease is gastric cancer.