Among these 20 key genes, five genes TWIST1 (45), SNAI2 (46), TSHR (47), FOXE1 (48), and CD274 (PD-L1) (49) have been reported to be differentially expressed in ATC compared to normal thyroid samples and other thyroid cancer samples both at mRNA and protein expression levels. The gene discussed is TWIST1; the disease is thyroid gland carcinoma.