A trend toward statistical significance between PD-L1 and histological types was also observed (Usual SCC vs. Others, OR = 1.754, 95% CI = 0.984-3.124, <i>P</i> = 0.070).<h4>Conclusions</h4>PD-L1 over-expression was related to worse survival outcomes and several clinicopathological features of penile cancer. This evidence concerns the gene CD274 and penile cancer.