GFAP and Alzheimer disease: Recapitulating the neuropathological features of human AD, co-immunostaining of GFAP and IBA1 reveals direct spatial associations of GFAP-positive astrocytes and IBA1-immunoreactive microglia with Aβ aggregates, with some of the activated astrocytes and microglia entangled with vascular structures harboring Aβ aggregates (Fig. 10c, d, g, h) indicating prominent angiopathies.