To confirm the biological significance of the correlations of these transcripts with sensitivity to CDK4/6 inhibitors, we constructed ENST00000257904 and ENST00000547281 transcript expression plasmids using the pCDNA3.1 vector (Fig. 6b), transfected the plasmids into MCF7 cells (a model of luminal A-type breast cancer, a subtype for which ribociclib treatment is used, Fig. 6c), and detected the sensitivity of the plasmid-transfected cells to ribociclib. The gene discussed is CDK4; the disease is breast cancer.