The findings from the current work included a) CLP from wild chlorella species (Chaetomorpha linum) contained mainly arabinogalactan sulfate; b) CLP treatment alleviated the symptoms of NAFLD, improved glucose intolerance and hyperlipidemia by enhancing the PPARα/CPT-1/MCAD signaling in mice after HFD induction. Here, PPARA is linked to metabolic dysfunction-associated steatotic liver disease.