SMAD3 and coronary artery disorder: MSP analysis and Sequenom MassARRAY analysis from 81 ACS samples, 74 stable coronary artery disease samples, and 53 healthy samples confirmed that the reference results of the HumanMethylation450 array significantly corrected the differential CpG methylation of SMAD3. These data identified ACS-specific DNA methylation profiles with several novel DM CpG sites, some of which could be candidate markers for early diagnosis of ACS [19].