The protein expression levels of TLR4, phospho-NFκB, p65, and pro-inflammatory cytokines, such as TNFα are dramatically increased in the brain of fetal mice by activating TLR4 signaling in microglia through maternal LPS treatment; these offspring show ASD-like behavior with less social behavior and increased anxiety and repetitive behaviors. The gene discussed is NFKB1; the disease is Anxiety.