Importantly, intrathecal injection of bombesin-saporin dramatically reduced c-Fos expression in the ACC (Fig. 1H, I), suggesting that the enhanced c-Fos labeling induced by DCP is indeed due to the presence of “itch”; this is also consistent with our previous work revealing the enhanced excitatory synaptic transmission of the ACC after chronic itch [48]. Here, GRP is linked to Pruritus.