Furthermore, combining our results with the mechanism of action of pyrotinib, inflammatory bowel disease, Toll-like receptor signaling pathway, IL-17 signaling pathway, ErbB signaling pathway, Pathogenic Escherichia coli infection, MAPK signaling pathway, cAMP signaling pathway and PI3k-Akt signaling pathway were chosen as potential pathways. Here, EGFR is linked to escherichia coli infection.