To test whether VLCFA treatment promotes chemokine production, as suggested by the whole transcriptome analysis of X-ALD macrophages (Fig. 1), we used RT-qPCR to analyse the expression of CXCL8, CCL3 and CCL4 in macrophages treated with C26:0 for up to 24 h. Here, CCL3 is linked to X-linked adrenoleukodystrophy.