Subsequently, FCM was used to detect the effect of Nrf2 on the sensitivity of AML cell lines to Ara-C, indicating that compared with the control (CON) and empty vector 1 (EV1) groups, Nrf2 upregulation in U937 and THP-1 cell lines could significantly reduce the apoptotic number of leukemic cells (Fig. 4F). This evidence concerns the gene NFE2L2 and acute myeloid leukemia.