To further investigate the biological function of SPTBN1 in ccRCC, we successfully established SPTBN1-knockdown and SPTBN1-overexpression cell models (786-O and Caki-1) and validated by qRT-PCR and WB (Additional file 1: Figure S8A, B, P < 0.05). This evidence concerns the gene SPTBN1 and nonpapillary renal cell carcinoma.