We obtained glioma stem cells (Supplementary Figure S10A) using specific media (DMEM/F12, with 2% B27, 25ng/mL bFGF, 25ng/mL EGF and 1% penicillin/streptomycin) and verified the changes in mRNA levels (CD133 U251 vs. GSCU251: P=0.0005; SOX2 U251 vs. GSCU251P=0.0034) and protein levels of their stem-biological markers (Supplementary Figure S10B). This evidence concerns the gene EGF and glioma.