To test the sensitivity of this functional assay, we generated TCR-transduced (Td) T cells specific for the previously described HLA-A*02:01-restricted HPV16 E711–19 epitope [4], which we isolated from a cervical cancer TIL culture with known specificity (Supplemental Figure 1b), spiked them into an healthy donor-derived CD8+ T cell fraction and tested them against K562 E7sh HLA-A*02:01 cells. The gene discussed is CD8A; the disease is cervical cancer.