Survival analysis on the basis of ANLN expression, which was conducted using the Kaplan–Meier method, in ESCC (GSE53625 dataset) showed that the overall survival of patients with high ANLN expression was lower than that of patients with low ANLN expression (Supplementary Fig. S1E, log-rank test, P = 0.0159). This evidence concerns the gene ANLN and esophageal squamous cell carcinoma.