To evaluate the function of vlPAG-DRGABA+ neurons on nociception and anxiety-like behaviors, we designed an experimental timeline (Fig. 1A) and applied an AAV carrying Cre-dependent hM3Dq/hM4Di or mCherry in the vlPAG-DR of GAD2-ires-Cre mice (Fig. 1B). The gene discussed is GAD2; the disease is Anxiety.