Gain‐ and loss‐of‐function experiments revealed that overexpression of FBP1 inhibited viability and colony forming capability and enhanced apoptosis in gefitinib‐resistant PC9/GR and HCC827/GR cells, while knockdown of FBP1 had the opposite effects, suggesting that FBP1 participated in gefitinib resistance in NSCLC cells. Here, FBP1 is linked to non-small cell lung carcinoma.