To examine CTL-mediated immunity during acute/early HIV infection, peripheral blood mononuclear cell (PBMC) samples collected from 11 acute/early-stage HIV infected subjects (n = six clade B, three clade AE, one clade C and one clade AG; Fig 1A) with known/inferred HIV transmission dates (acute WA cohort; Table 1) were assessed for IFNγ responses following stimulation with individualized peptide sets. Here, IFNG is linked to HIV infectious disease.