To be more specific, TNFSF4, CD86, PDCD1LG2, TNFRSF4, HAVCR2, CD28, TNFSF18, TNFRSF9, PTPRC, PDCD1, IL12B, CD80, JAK1were significantly highly expressed in gastric tumor tissues and cluster II that associated with worse OS. This evidence concerns the gene CD28 and gastric neoplasm.