GSDME and neoplasm: For example, chimeric antigen receptor (CAR) T cells from NK cells and CD8+ T lymphocytes released GzmB into tumor cells in a perforin (PFN)-dependent manner, and then GzmB directly processed GSDME after D270 residue, a site also cleaved by caspase-3, releasing cytotoxic GSDME-NT to form pores in plasmalemma (16).