We assessed the Casp1 KO effects on renal fibrosis initially by collagen (type1-α1 and type III-α1) staining of sections from fixed samples with picrosirius red (Figure 4F) Because of the predominantly female effect of Casp1 KO on cyst number and kidney size, we concentrated this analysis on female samples. This evidence concerns the gene CASP1 and renal fibrosis.