CASP3 and hepatocellular carcinoma: Supplementary Figure S1B shows that FOD could also induce apoptosis in mouse HCC cells H22. Hoechst33258/PI staining was used to detect apoptotic HepG2 cells. There were almost no apoptotic cells in the control group, but atrophic, hyperchromatic and pyknotic nuclei were observed in the FOD group (Figure 4B). We next studied cleaved caspase-3 expressions in HepG2 cells treated with different concentrations of FOD using Flow cytometry. As shown in Figure 4D, FOD treatment elevated the expression of cleaved caspase-3 protein.