In this study, we used ELISA to detect the contents of cytokines IL-1β, IL-6, TNF-α, IL-12, and TGF-β in mouse intestinal tissue, and we found that QYJD could significantly reduce the contents of IL-1β, IL-6, TNF-α, and TGF-β in colon tissue of mice with colitis. The gene discussed is TNF; the disease is colitis.