To determine the downregulation of PD-L1 expression under AIL treatment through c-Jun inhibition, we overexpressed c-Jun in SK-MEL-5, SK-MEL-28, B16-F10 and YUMM1.7 melanoma cell lines (Fig. 5K, Additional file 2: Fig. S7E and Fig. S8E). The gene discussed is JUN; the disease is melanoma.