SLC4A4 and pancreatic neoplasm: First, we generated mouse Panc02 and KPC pancreatic cancer cells engineered with a doxycycline-inducible CRISPR–Cas9 system and either a single guide RNA (sgRNA) targeting Slc4a4 (sgSlc4a4) or a non-targeting guide RNA (gRNA; sgNT) as a control (Extended Data Fig. 2a–c).