Despite the reinvigorated antitumoral immune response after Slc4a4 targeting, in the Panc02 model, expression of programmed cell death protein 1 (PD-1) in CD8+ T cells and cytotoxic T lymphocyte-associated protein 4 (CTLA-4) in Treg cells (considered the most representative cellular compartments for the expression of these immune checkpoints45) and programmed death ligand 1 (PD-L1) levels in cancer cells in vivo were still high and comparable in sgNT versus sgSlc4a4 Panc02 tumors (Fig. 8a–c, left, and Extended Data Fig. 8a). This evidence concerns the gene PDCD1 and cancer.