To examine the role of AKAP8L in the stemness of gastric cancer cells, BGC-823/Oxa and MKN-45/Oxa cells were transfected with AKAP8L overexpression or AKAP8L short hairpin (sh)RNA letivirus (Fig. 2A) and grew them under spheroid-formation conditions. Here, AKAP8L is linked to gastric cancer.