The results of the Western blot analysis showed the presence of strong bands at the predicted molecular weight for α2δ-1, TLR-4, and Sp1 (~150, ~95, and ~90 kDa, respectively) in the lysates of the two cell lines studied (S1 Fig), corroborating the expression of these three proteins in U87 human glioblastoma cells. This evidence concerns the gene TLR4 and glioblastoma.