To study the role of the CaV channel α2δ-1 subunit in the proliferative and migratory capacity of U87 cells, and whether the regulation of its expression was dependent on Sp1 after activation of TLR-4, we initially investigated the expression of these proteins in the cell line by Western blot, using antibodies available from commercial sources (see Material and Methods Section) and the human neuroblastoma SHSY-5Y cells as a positive control. Here, SP1 is linked to neuroblastoma.