TLR4 and glioblastoma: Fig 1B shows a representative image of a semiquantitative Western blot experiment, where a higher density band is observed in the lysates from U87 cells treated with LPS compared to the loading control (β-actin). Last, Fig 1C shows that, on average, the level of α2δ-1 protein expression was ∼1.8-fold higher in LPS-treated U87 human glioblastoma cells compared to untreated controls. These data suggest that the activation of TLR-4 by LPS may trigger signaling pathways that positively regulate the expression of α2δ-1.