Immunostaining of the tissue samples from the 47 participants (S4 Table) revealed that the rate of positive AIF-1 expression was significantly higher for patients with lymph node metastasis (P < 0.05 vs. no lymph node metastasis) TNM stage III–IV (P < 0.05 vs. TNM stage 0–II), and positivity for Ki67 expression (P < 0.05 vs. no Ki67 expression). This evidence concerns the gene AIF1 and metastatic malignant neoplasm in the lymph nodes.