Using sc-RNA-Seq and genomic data analysis, we show that cocultured EC and FB progressed to cell phenotypes that are also enriched in patients with IPF, and we identified expression of NF-κB and AP-1 gene modules in distinct cell populations of patients with IPF, highlighting the translational potential of our model. This evidence concerns the gene NFKB1 and idiopathic pulmonary fibrosis.