To investigate whether Tiam1 is activated in the ACC of mouse models of chronic pain displaying depressive/anxiety-like behaviors, we carried out an affinity-precipitation assay to detect active Tiam1 using GST-Rac1G15A, a guanine nucleotide–free form of Rac1 that preferentially binds to activated GEFs (20). The gene discussed is RAC1; the disease is Anxiety.