Subsequently, normal breast epithelial cell line (MCF-10A) and three breast cancer cells with different receptor expressed (ER+ BC cell line MCF-7, HER2+ BC cell line BT474 and triple-negative breast cancer (TNBC) cell line BT549) were chosen to detect cuproptosis-related gene expression via RT-qPCR experiments, suggesting that the expression levels of MTF1 and PDHB were significantly higher in three above kinds of BC cells than in MCF-10A, whereas DLD and DLAT were higher expressed in MCF-7 and BT-474 than in MCF-10A. The gene discussed is MTF1; the disease is triple-negative breast carcinoma.