However, in the group administered with LETZ+GA, we observed significant reduction (P < 0.001) in serum testosterone, LH and LH/FSH levels as well as significant increase (p < 0.001) in estrogen and FSH levels as compared to the LETZ-induced PCOS group (Fig. 4a-c). This evidence concerns the gene PLOD1 and polycystic ovary syndrome.