COL28A1 and renal fibrosis: As can be seen from the Masson staining in Figure 3(b), the area dyed blue in the UUO + COL28 overexpression group was significantly elevated relative to the UUO + COL28-NC group, suggesting that the degree of renal fibrosis was aggravated; that is, the overexpression of COL28 in the renal tissue of UUO mice could aggravate renal interstitial fibrosis.