The two 8-color pretitred tubes, containing the markers for the recognition of plasma cells, are constructed to perform a sequential gating based on the recognition of the backbone markers (CD45, CD19, CD38, CD138) and the expression of the additional markers that could be aberrantly present on MM PCs surface (CD56, B2, CD117, CD81, CD27, CD28). The gene discussed is KIT; the disease is Miyoshi myopathy.