Microglia were pharmacologically depleted in 6-week diabetic CX3CR1-WT mice using PLX-5622 and analyzed at three different timepoints: (1) at 6 weeks of diabetes (6D) to establish the retinal transcriptome prior to microglia depletion; (2) after 2 weeks microglia depletion (PLX-treatment), and (3) after 2 weeks of microglia repopulation (PLX-recovery) (Fig. 6A). The gene discussed is CX3CR1; the disease is diabetes mellitus.