The most significant genes in RNA sequencing included upregulation of MAPK effectors Maff (P < 0.0001), Rassf1 (P < 0.0001), JunD (P < 0.0001), Atf4 (P < 0.0001), Dusp8 (P < 0.01), Dusp14 (P < 0.01) and Fosl2 (P < 0.01) in LPD which were confirmed by qRT-PCR (Fig. 2A). This evidence concerns the gene DUSP8 and disseminated peritoneal leiomyomatosis.