Next in the pathway, phosphofructokinase (Pfk) isoforms, responsible for F6-P breakdown, were unchanged in RNA sequencing but in qRT-PCR three of seven Pfk isoforms tested showed divergent changes, either increased (PfkM; P < 0.05) or decreased (PfkP; P < 0.01; PfK4; P < 0.1) expression in LPD (Fig. 3D). The gene discussed is PFKM; the disease is disseminated peritoneal leiomyomatosis.