Regardless, shRNA knockdown experiments with validated constructs against SOX4 (three constructs) and BACH2 (one construct) did not yield consistent results in human AML cell lines with recombined MLL locus (MV-4-11, THP-1, and NOMO-1) (Fig. S7A, C and data not shown). This evidence concerns the gene KMT2A and acute myeloid leukemia.