irg1 has been found to be expressed specifically in zebrafish macrophages and is upregulated during infection.41,42 We then crossed this line with the vegfaa reporter line TgBAC(vegfaa:eGFPpd260)43 (vegfaa:eGFP throughout) and infected double transgenic irg1:tdTomato; vegfaa:eGFP progeny with M. marinum expressing eBFP2 (Mm-eBFP2) to simultaneously visualize bacteria, macrophage localization, and vegfaa production in vivo. The gene discussed is ACOD1; the disease is infection.