Collectively, these in vivo results showed that Apoptin caused significant changes in the expression of AIF, HtrA2, Smac/Diablo, and Cyto‐C and that BAPTA‐AM could reduce the expression of Smac/Diablo and Cyto‐C in Apoptin‐treated tumor tissues. The gene discussed is AIFM1; the disease is neoplasm.