In the present study, we mainly focused on the role of TMEM100 in the AEW itch model and the regulatory mechanism of TMEM100 in TRPV1 and TRPA1 functional changes to clarify the mechanism of the TRPV1 and TRPA1 functions modulated by TMEM100 during dry skin-induced itch. The gene discussed is TRPA1; the disease is Pruritus.