In this study, we comprehensively investigated the association between biomarkers of iron metabolism, including SI, SF, TSAT, and sTfR, and the prevalence of NAFLD in the general population of the U.S., utilizing data from the National Health and Nutrition Examination Survey (NHANES) collected from 2017 to 2018. This evidence concerns the gene TFRC and metabolic dysfunction-associated steatotic liver disease.