In this study, we identified hsa_circ_0060927 (originate from the back splicing of cytochrome P450 family 24 subfamily A member 1, also known as circCYP24A1) in ESCC tissues by RNA sequencing, which is up-regulated in ESCC tissues and acts as a novel prognosis-related factor in ESCC. The gene discussed is CYP24A1; the disease is esophageal squamous cell carcinoma.