Furthermore, CCK-8 and Edu assays indicated that the proliferation ability was significantly reduced in the ESCC cells with SCARA5 overexpression, and the flow cytometry assay of cell cycle revealed that the cell cycle was arrested in G0/G1 phase of the ESCC cells with SCARA5 overexpression. The gene discussed is SCARA5; the disease is esophageal squamous cell carcinoma.