GSEA of the top 100 genes identified the KRAS, TP53, TNFA via NFKB, and IL6/STAT3 signaling pathways as enriched in genes differentially expressed in HCC tumor cells (P value < 0.05), very similar to the bulk RNA sequencing analysis (Fig. 5b). The gene discussed is NFKB1; the disease is hepatocellular carcinoma.