We screened a series of individual TIL cultures isolated from 2 to 4 mm tumor fragments from a patient (Hu-56) with HPV-16+ head and neck squamous cell carcinoma (HNSCC) (Supplemental Table 1; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.165570DS1) by IFN-γ ELISPOT assays to identify T cell responses directed against HPV-16 viral oncoproteins E6 and E7, using peptide pools comprising overlapping 15-mers spanning the length of the E6 and E7 proteins. Here, IFNG is linked to neoplasm.