HLA-DRB5 and familial pancreatic carcinoma: To generate HLA-matched MHC-II+ cell lines for these studies, we transduced the KRASG12V+ cell lines NCI-H2444 and DAN-G, derived from human lung and pancreatic cancers, respectively, with CIITA and a construct encoding HLA-DRB5*01:01 with a truncated CD34 reporter gene (Figure 6A).